Construction and Analysis of SSH Cdna Library of Solanum Torvum Roots Infected by Verticillium Dahliae Kleb.

Verticillium wilt caused by the fungus Verticillium dahliae Kleb.is a serious soilborne disease in eggplant.The eggplant cultivars are susceptible,but wild Solanum torvumis highly resistant to Verticilli um wilt.A forward suppression subtractive hybridization library was established using roots of S.torvum,and the cDNAs from the fungus inoculated seedlings were used as the tester and the normal uninoculated seedlings as the driver.(1)170positive clones were randomly selected and sequenced,and 109non-redundant ESTs were obtained,among which 61ESTs share sequences homology with stress resistance related genes of other plants.Such as chitinase,cytochrome P450,peroxidase,protease inhibitorⅡ,etc.Moreover,a EST in SSH was used as a querying probe to blast the EST database in GenBank.(2)Based on the assembled homologous cDNA sequences and RT-PCR,a universal stress gene was cloned,designated StUSP1.(3)Expression pattern revealed that StUSP1was up-regulated in roots while S.torvum was infected by Verticillium wilt.This SSH library is helpful to understand the resistance mechanism of wild eggplant to Verticillium wilt.