Selection of Virus Strains Used for HFRS Purified Divalent Vaccine and Study It's Immunogenecity

The sukling mice were infected with LR 1 and R 22 viral strains by inoculation in brain and in peritoneal cavity then adapted in Vero cell culture.The parameters such as amount of antigen,virulence titer of virus strains were also under the investigation.Apparently the parameters of adapted ones were higher than those of direct cell propagated.The monovalent and purified divalent vaccines were prepared from virus strains cultured Vero cells,which were used for preparation of rabit antiserum.As a consequence,the neutralizing antibody was detected by PRNT.The results showed that the adapted LR 1 and R 22 had excellent immunogenicity for vaccine preparation.