Stem cells derived from human exfoliated deciduous teeth-based media in a rat root resorption model

Objective: Root resorption may occur during orthodontic treatment. Herein, we investigated the effect of a culture supernatant of stem cells derived from human exfoliated deciduous teeth on root resorption.Design: Twelve 8-week-old male Sprague-Dawley rats were used, and their maxillary first molars were pulled with excessive orthodontic force to induce root resorption. On days 1 and 7 after traction initiation, stem cells derived from human exfoliated deciduous teeth and alpha minimum essential medium (control group) were administered. After 14 days, the maxillary bone was evaluated for tooth movement. The expression of osteoprotegerin, receptor activator of nuclear factor kappa B ligand, tumor necrosis factor alpha, interleukin 1 beta, interleukin 6, and interleukin 17 was evaluated on the compression side and tension side. Results: No significant difference in tooth movement was observed between the two groups. Root resorption decreased in the group administered the culture supernatant compared with in the control. Immunohistochemical staining revealed increased osteoprotegerin expression and decreased receptor activators for nuclear factor kappa B ligand, tumor necrosis factor alpha, interleukin 1 beta, interleukin 6, and interleukin 17 on the compression side and tension side.Conclusions: Administration of stem cells derived from human exfoliated deciduous teeth affected the expression of osteoprotegerin, receptor activator of nuclear factor kappa B ligand, tumor necrosis factor alpha, interleukin 1 beta, interleukin 6 and interleukin 17; hence, these stem cells may inhibit root resorption by regulating their expression.