Cannabis Sativa Mediated Palladium Nanoparticles As an Effective Nanodrug Against Multi-Drug Resistant Bacteria and A549 Lung Cancer Cells

The present paper reports a biogenic synthesis of palladium nanoparticles (PdNPs) involving Cannabis sativa leaf extract. The physicochemical characterisations of biogenic synthesised PdNPs were carried out to determine size, shape, morphology, atomic composition, and crystal structure via different techniques involving Ultraviolet-visible (UV-vis), Fourier transform infrared (FT-IR), Field emission scanning electron microscopy (FESEM), Transmission electron microscopy (TEM), and X-ray diffraction (XRD). The standard particle size of the synthesized PdNPs was found to be 3.49 +/- 0.5 nm from the TEM analysis, while XRD analysis showed a crystal size of 4.6 nm with a face-centred-cubic (fcc) structure. FT-IR study of the green synthesis confirmed the presence of phytochemicals which act as reducing and stabilising agents. The synthesised biogenic PdNPs were subjected to antimicrobial analysis for isolated strains of multi-drug resistant (MDR) Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Salmonella abony where minimum inhibitory concentration (MIC) was found in the range of 52.04-68.3 mu g/mL. Moreover, for the cytotoxic effects of biogenic PdNPs, A549 lung cell lines were used, and the half-maximal inhibitory concentration (IC50) value was evaluated as 22.17 mu g/mL. The present investigation suggests the possible role of C. sativa extract capped PdNPs in the clinical management of lung cancer cells and MDR pathogens.