PI-(3,5)P2-mediated oligomerization of the endosomal sodium/proton exchanger NHE9

Na+/H+ exchangers are found in all cells to regulate intracellular pH, sodium levels and cell volume. Na+/H+ exchangers are physiological homodimers that operate by an elevator alternating-access mechanism. While the structure of the core ion translocation domain is fairly conserved, the scaffold domain and oligomerization show larger structural variation. The Na+/H+ exchanger NhaA from E. coli has a weak oligomerization interface mediated by a β-hairpin domain and homodimerization was dependent of the lipid cardiolipin. Organellar Na+/H+ exchangers NHE6, NHE7 and NHE9 are likewise predicted to contain β-hairpin domains and a recent analysis of horse NHE9 indicated that the lipid PIP2 binds at the dimerization interface. Despite predicted lipid-mediated oligomerization, their structural validation has been lacking. Here, we report cryo-EM structures of E. coli NhaA and horse NHE9 with the coordination of cardiolipin and PI(3,5)P2 binding at the dimer interface, respectively. Cell based assays confirms that NHE9 is inactive at the plasma membrane and thermal-shift assays, solid-supported membrane (SSM) electrophysiology and MD simulations, corroborates that NHE9 specifically binds the endosomal PI(3,5)P2 lipid, which stabilizes the homodimer and enhances activity. Taken together, we propose specific lipids regulate Na+/H+ exchange activity by stabilizing oligomerization and stimulating Na+ binding under lipid-specific cues. ### Competing Interest Statement The authors have declared no competing interest.