Polished hollow core Bragg fiber sensor for simultaneous measurement of cortisol concentration and temperature.

Disturbance of surrounding temperature inevitably affects the accuracy of fiber biosensors. To that end, we propose a compact label-free optofluidic sensor based on a polished hollow core Bragg fiber (HCBF) that can simultaneously measure the cortisol concentration and surrounding temperature in real-time. The sensor is comprised of fusion splicing single mode fiber (SMF), multimode fiber (MMF) and HCBF. HCBF is side polished to remove part of the cladding that the suspended inner surface of the fiber can contact the external environment. After the incident light passes through the MMF from the SMF, it enters the hollow area, high refractive index (RI) layers, respectively, where the anti-resonant reflecting optical waveguide (ARROW) guiding mechanism and Mach-Zehnder interferometer (MZI) are simultaneously excited. Taking advantage of the high RI layers of HCBF, compared to the fiber with uniform cladding, the light can be more confined in the cladding and more sensitive to inner surface medium. The inner surface of sensor is immobilized with cortisol aptamer for the sake of achieving high sensitivity and specific sensing of cortisol with the limit of detection (LOD) to be 4.303 pM. The proposed sensor has a compact structure, enables temperature compensation, and can be fabricated at low cost making it highly suitable for in-situ monitoring and high-precision sensing of cortisol and other biological analytes.