Selective Adsorbents Based on Imprinted Glucose Oxidase

A procedure for the synthesis of a highly selective adsorbent based on an imprinted protein (IP), glucose oxidase (GO), and commercially available SiO2 microparticles for the sorption of mycotoxin zearalenone produced by fungi of the Fusarium species is developed. The use of 3D fluorescence spectroscopy for the control of the process of IP purification is proposed for the first time. A possibility of replacing the zearalenone molecule as a template molecule with structural analogues with lower toxicity is assessed. The analytical characteristics of the determination of zearalenone using imprinted GO as a receptor element in enzyme immunoassay are determined: the limit of detection is 5 ng/mL, the linear analytical range is 11–112 ng/mL. High sorption characteristics of the synthesized adsorbent based on IP (sorption capacity—7.6 μg/mg sorbent; imprinting factor—2.5) are shown.