Evaluation and selection of antibodies suitable for endoglin detection to be applied in the study of liver and aorta pathology on mice models

Background and Aims: Endoglin (CD105, Eng) is a glycoprotein affecting the function of vascular endothelium and liver fibrosis. If immunoassay techniques that use antibodies for the specific detection of proteins are involved, finding a high-quality antibody is crucial. We found that some commercial anti-Eng antibodies show contradictory results regarding Eng expression during the development of atherosclerosis and non-alcoholic steatohepatitis (NASH). We aimed to evaluate the affinity and specificity of these antibodies and suggest the most suitable antibody detecting Eng in the aorta and liver in these pathologies.