Research on Apoptosis of K562 Cells Induced by a Novel Nitrogen Mustard Derivative XHH

OBJECTIVE To investigate the antitumor effects and its possible mechanisms of a novel nitrogen mustard derivative(XHH) on K562 cells.METHODS Cell proliferative effect was assessed by MTT assay.The changes of morphology,apoptotic rate and mitochondrial membrane potential(MMP) were assessed by AnnexinV-FITC/Hoechst33342,PI/Hoecsht33342 and Rh123/Hoechst33342 double staining using high content screening(HCS),respectively.The activation of caspase-3,caspase-9 was evaluated by spectrophotometric method.RESULTS XHH showed a better proliferation inhibition effection on K562 cells than Melphalan due to its induction of apoptosis in a dose-and time-dependent manner,reduction of MMP,and activation of caspase-3 and caspase-9.CONCLUSION XHH showed a nice antitumor activity,induced apoptosis of K562 cells via caspase-9/caspase-3 mitochondrial pathway.