A time-resolved clonogenic assay for improved cell survival and RBE measurements.

Robin A Koch,Marc Boucsein,Stephan Brons,Markus Alber,Emanuel Bahn

Clinical and translational radiation oncology（2023）

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摘要

The IVCA suffers from substantial uncertainty caused by the overlap of size distributions of delayed abortive and clonogenic colonies. This impairs precise quantification of cell survival and RBE. By considering colony growth over time, our method improves assaying clonogenicity.

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关键词

Radiobiology,Dose-response relationship, radiation,Relative biological effectiveness,Heavy ion radiotherapy,Time-lapse imaging,Supervised machine learning

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