The effect of dietary supplementation of proteases on growth, digestive enzymes, oxidative stress, and intestinal morphology in fishes - A review

Optimizing utilization of nutrients and improving protein digestibility, particularly in the early stages of cultivable species, is a challenging task for the sustainable development of aquaculture. The use of protein-digesting enzymes as a feed supplement is an eco-friendly approach to improve protein digestibility. Exogenous enzymes, particularly proteases, have been shown to improve fish growth, nutrient digestibility, immunological parameters, intestinal topography, and most importantly, water quality. Although there is a clear understanding of the role of proteases and their mechanism of action, their role as a feed supplement in aquaculture remains controversial. In this review, we present updated information on the impact of proteases in improving various development indicators and fish health in general. Based on the information gathered from the current state of knowledge, it is clear that extensive research has to be made to broaden the application of proteases overcoming the drawbacks that could hinder its application in the field.