Running Title: P. Falciparum Gametocyte-Specific Antibody Profiling

Plasmodium falciparum gametocyte-specific antibody profiling reveals boosting through 1 natural infection and identifies potential markers of gametocyte exposure 2 3 Running title: P. falciparum gametocyte-specific antibody profiling 4 5 Jeff Skinner, Chiung-Yu Huang, Michael Waisberg, Philip L. Felgner, Ogobara K. 6 Doumbo, Aissata Ongoiba, Kassoum Kayentao, Boubacar Traore, Peter D. Crompton#, Kim 7 C. Williamson# 8 9 Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National 10 Institutes of Health, Rockville, Maryland, USA 11 Division of Biostatistics and Bioinformatics, Sidney Kimmel Comprehensive Cancer Center, 12 Johns Hopkins University, Baltimore, Maryland, USA 13 c Division of Infectious Diseases, Department of Medicine, University of California, Irvine, CA 14 92697, Antigen Discovery, Inc., Irvine, CA 92618 15 Mali International Center of Excellence in Research, University of Sciences, Techniques and 16 Technologies of Bamako, Bamako, Mali 17 Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious 18 Diseases, National Institutes of Health, Bethesda, MD 20852, Department of Biology, Loyola 19 University Chicago, Chicago, IL 60660 20 #Co-corresponding authors: Peter D. Crompton, pcrompton@niaid.nih.gov, and Kim C. 21 Williamson, kwilli4@luc.edu. 22