A visual assay panel for the identification of monkeypox virus DNA belonging to the clades I and II.

•A MPXV visual assay panel is a rapid and reliable tool to differentiate the clades I and II within 25 min.•This panel combines RAA and immunochromatography, and detects as low as 1 copy/μL recombinant plasmid.•Visual assay panel shows no cross-reactivity with orthopoxviruses and herpesvirus that infect humans, such as vaccinia virus.