Practical Considerations for Accurate Determination of Free Thyroxine by Equilibrium Dialysis.

Ashley Ribera,Li Zhang,Carla Ribeiro,Norma Vazquez,Janet Thonkulpitak,Julianne C. Botelho,Uliana Danilenko,Katleen van Uytfanghe,Hubert W. Vesper

JOURNAL OF MASS SPECTROMETRY AND ADVANCES IN THE CLINICAL LAB（2023）

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Abstract
•Pre-analytical factors of FT4 measurement can influence method quality.•Equilibrium dialysis device choice can lead to variability among FT4 methods.•Labware properties can negatively impact concentration of thyroxine solutions.•Solvent composition can shorten the stability of neat T4 solutions during storage.
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Key words
ED,FMED,HEPES,IA,IFCC,IS,LC-MS/MS,MIT,MWCO,qNMR,RMP,SPE,WS
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