RNAscope™ Multiplex Detection in Zebrafish.

The RNAscope methodology is a powerful tool to detect RNA expression patterns with high subcellular resolution and possibility for RNA-protein colocalization studies. Presented here is a two-day protocol for robust multiplex detection of up to three different RNAs in zebrafish whole-mount embryos using the RNAscope procedure. Application of the protocol offers the simultaneous detection of multiple RNAs with a high signal-to-noise ratio in an intact embryo.