The efficacy and mechanism of platelet-rich plasma on the myogenic differentiation process of adipose-derived stem cell: A bioinformatics and experimental study

Abstract Background: The adipose-derived stem cells (ADSCs) have been proved to be effective in the myogenic differentiation with the 5-Aza induction. However, the induction procedure definitly decreased the proliferation ability of ADSCs. The platelet-rich plasma (PRP), famous of its proliferating abilities, is widely used in the field of tissue regeneration. The purpose of this study is to determine the efficacy and mechanism of PRP on the myogenic differentiation process.Method: The published gene expression profile, GSE 70918, which compared the efficacy of PRP and platelet-rich plasma (PPP) on the tendon fibroblast was obtained from the Gene Expression Omnibus (GEO) database.The further protein‑protein interaction (PPI) network Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was performed based on differentially epressed genes(DEGs). Furethermore, the micro-RNA-mRNA interaction network was analyzed and visualized by cytoscape. Finally, the in vitro experimental cell research was performed to certify the efficay of PRP on myogenic differentiation.Results: From our analysis, 148 DEGs, 87 up-regulated and 61 down-regulated, were obtained from the gene profile. The PPI network showed that the inflammatory factors as IL-1A, CXCL, CXCR etc. were significantly down-regulated and the muscle functional related genes as MYLC and MYO7A were significantly up-regulated. The GO and KEGG analysis demonstrated that up-regulated DEGs were enriched in focal adhesion, dilated cardiomyopathy, hypertrophic cardiomyopathy and calcium signaling pathway which were correlated to muscle function. Meanwhile, the microRNA-mRNA interaction network were estabilished based on DEGs. The in vitro experimental showed that PRP can significantly increase the proliferation ability of ADSCs during myogenic introduction and increase the expression of troponin and myosin which were correlate to the function of mature muscle.Conclusions:The research showed that PRP is an effective way to enhance the proliferation ability during the myogenic differentiation and increase the expression of muscle functional related protein.