Mechanism of Mir-590-3p Carried by Tumor-Derived Extracellular Vesicles in Promoting Invasion and Metastasis of Ovarian Cancer

Background Ovarian cancer (OC) remains a common gynecologic malignancy. Tumor-derived extracellular vesicles (EVs) contribute to pro-metastasis microenvironment by carrying microRNAs (miRs). This study investigated the mechanism of miR-590-3p carried by OC cell-derived EVs in OC metastasis. Methods miR-590-3p expression in OC tissues and cells was measured. EVs were extracted from healthy serum and the serum of patients with OC or metastatic OC. EVs were extracted from OC cells and normal OC epithelial cells in vitro. miR-590-3p expression in EVs was tested. The effect of EVs-miR-590-3p on the proliferation, migration and invasion of OC cells was measured. The target of miR-590-3p was predicted and verified. The effect of miR-590-3p targeting CPEB3 on OC cells was confirmed by functional rescue assays. Xenograft tumor experiment was performed to verify the mechanism of EVs-miR-590-3p in the tumorigenesis and metastasis of OC. Results miR-590-3p expression was enhanced in OC, and correlated with OC metastasis. miR-590-3p was elevated in OC cell-derived EVs and could be transferred to other OC cells by EVs. OC cell-derived EVs facilitated proliferation, invasion and migration of OC cells by transferring miR-590-3p. miR-590-3p targeted CPEB3. Overexpressing CPEB3 repressed the promoting effect of EVs-miR-590-3p on OC cells. In vivo experiment confirmed that EVs-miR-590-3p facilitated tumorigenesis and metastasis of OC cells by targeting CPEB3. Conclusion OC cell-derived EVs facilitated progression and metastasis of OC via the miR-590-3p/CPEB3 axis.