Large-Scale Quantitatively Detecting and Analyzing of ceRNAs reveal NOTCH3 mRNA, miR-369-3p and rno-Rmdn2_0006 Together Regulate the Hepatocytes in G0 Phase and in G1 Phase During the Initiation Stage of Rat Liver Regeneration

Abstract Aims:Generally, key events of the liver regeneration initiation (LRI) are how the hepatocytes in G0 phase change to G1 phase. We use the data from large-scale quantitatively detecting and analyzing (LQDA) to reveal ceRNAs together regulate the hepatocytes in G0 phase or in G1 phase during LRI.Main methods:The hepatocytes of the rat liver right lobe were isolated at 0 hour, 6 hour and 24 hour after partial hepatectomy (PH), their ceRNA expression abundance was measured by LQDA, and the correlation of their expression, interaction and role were revealed by ceRNA comprehensive analysis. Key findings:It was found that expression of NOTCH3 mRNA is up-regulated at 0 h, but miR-369-3p and rno-Rmdn2_0006 of hepatocytes not change significantly at that, meanwhile, the G0 phase-related gene CDKN1c promoted by NOTCH3 up-regulation, and the G1 phase-related gene PSEN2 inhibited by NOTCH3 down-regulation at that. On the contrary, NOTCH3 mRNA and rno-Rmdn2_0006 were up-regulated at 6 h, but miR-136-3p was down-regulated at that, the G1 phase-related genes CHUK, DDX24, HES1, NET1 and STAT3 promoted by NOTCH3 up-regulated, and the G0 phase-related gene CDKN1a inhibited by NOTCH3 down-regulated at that. These results suggested that the ceRNAs and the NOTCH3-regulated G0 phase- and G1 phase-related genes show a correlation in expression, interaction and role, they together regulate the hepatocytes in G0 phase at 0 h and in G1 phase at 6 h. Significance:These discovers are helpful to understand the mechanism which ceRNA together regulate the hepatocytes in G0 phase or G1 phase.