Metagemonic analysis of bacterial genetic diversity in blood samples of Iranian healthy women

Abstract Rising evidence proved the existence of dormant blood microbiota in healthy individuals, yet there is no information on the circulating microbiome of Iranian healthy women. The High-throughput Next-Generation Sequencing (NGS) provides a powerful method for the characterization of the blood microbiome. We investigated circulating bacterial composition in healthy individuals by 16S rDNA- based next-generation sequencing technique targeting the V3-V4 hypervariable region on the Illumina platform. We identified Operational Taxonomic Units (OTUs) similarity with various phyla, predominated by Proteobacteria (83%), Firmicutes (8%), Bacteroidetes (6%), and Actinobacteria (2%) phyla in healthy subjects. Based on the sequencing data, a diverse bacterial family was found in the whole blood of the healthy subjects, belongs to Burkholderiaceae (69%) and Enterobacteriaceae (13%) families on average. At the genus level, we illustrated the abundance of Ralstonia, Rhizobium, Ignatzchineria, Lactococcus, Stenotrophomonas, and Ideonella. Given the dominance of the putative blood microbiota within the different niches, it may be of considerable clinical significance and therapeutic strategies potential in the future. The blood microbiota can facilitate both the diagnosis and improved understanding of the onset of numerous human diseases as innovative biomarkers.