Sensitive detection of fat mass and obesity-associated protein based on terminal deoxynucleotidyl transferase-mediated signal amplification

Schematic presentation of Sensitive Detection of Fat Mass and Obesity-Associated Protein (FTO). FTO can specifically demethylate the substrate probe, and the emerging double strands can be cleaved by DpnII which is a methylation-sensitive restriction endonuclease. The polymerase TdT in turn recognizes the free 3′OH and initiates polymerization to produce a mass of G-quadruplexes. Thioflavin T (ThT) can then be embedded into G-quadruplexes, producing a strong fluorescence.