A PAPER-BASED DUAL APTAMER ASSAY ON AN INTEGRATED MICROFLUIDIC SYSTEM FOR DETECTION OF HNP 1 AS A BIOMARKER FOR PERIPROSTHETIC JOINT INFECTIONS

This work demonstrated a novel dual-aptamer assay performed on a paper-based membrane composed of nitrocellulose (NC) on an integrated microfluidic platform for detection of a biomarker, human neutrophil peptide 1 (HNP 1) for periprosthetic joint infection. A fully automated device involved a single loading process (< 5 min) for the newly developed sandwich assay for HNP 1 quantification. The primary aptamer was immobilized on NC membrane where HNP 1 was captured and detected using fluorescent-labelled secondary aptamer. The developed assay is faster (similar to 30 min) and required less volume (only 50 mu L) than traditional assays.