Comparison of Collagens Extracted from Swim Bladder and Bovine Achilles Tendon

Collagen is a type of natural biopolymer material, which is widely used in tissue engineering and medicine owing to its exceptional properties such as biodegradability, biocompatibility, hemostatic properties, and low immunogenicity. Collagens from different sources can differ in type, structure, and function. In this study, collagen was extracted from swim bladder and bovine Achilles tendon by acid-enzyme binding method at low temperature. UV spectrum, Fourier transform infrared spectrum, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, scanning electron microscope, and differential scanning calorimetry were used to characterize these two collagens. The blood compatibility and cytotoxicity of the two kinds of collagen were studied.The results showed that the collagens from the two sources belong to the characteristics of type I collagen and had biological safety. Their differences in structure and thermal stability can provide a theoretical basis for the selection of collagen in practical application.