Brumimicrobium oceani sp. nov., isolated from coastal sediment saline lake and Genome Insight of the Genus Brumimicrobium

Abstract A novel Gram-stain-negative, aerobic, non-motile, rod-shaped and orange-colored bacterium, designated as strain C305T, was isolated from marine sediment of the coast area of Weihai, China. Strain C305T growth occurs at 4–40 °C (optimally at 30–33 °C), pH 6.0–9.0 (optimally at pH 8.0) and with 0.5–10.0 % (w/v) NaCl (optimum 1.5–3.0 %). No growth is observed without NaCl. The major cellular fatty acids of strain C305T were identified as iso-C15:0 ，iso-C15:1G and iso-C17:0 3-OH. The major respiratory quinone was found to be MK-6, and the DNA G+C content was determined to be 35.5mol %. The predominant polar lipids were mainly Phosphatidylethanolamines (PE), unidentified aminophospholipids (APL), and a kind of unidentified lipid (L2). Phylogenetic analysis based on 16S rRNA gene sequences revealed that C305T was a member of the genus Brumimicrobium and had a 16S rRNA gene sequence similarity values of 96.9–98.0 % with recognized Brumimicrobium species. On the basis of the phylogentic and phenotypic evidences, strain C305T represents a novel species of the genus Brumimicrobium, for which the name Brumimicrobium oceani sp. nov. is proposed. The type strain is C305T (= KCTC 62371T =MCCC 1H00297T).