Supplemental Methods from Downregulating Neuropilin-2 Triggers a Novel Mechanism Enabling EGFR-Dependent Resistance to Oncogene-Targeted Therapies

This document describes Materials and Methods applied for siRNA, shRNA, and cDNA transfer in mammalian cells, by means of transfection or lentiviral-mediated cell transduction. Moreover, supplementary information are provided about genomic DNA and RNA isolation and Real-time quantitative PCR analysis, as well as gene promoter reporter assays. Finally, cell surface protein-biotinylation and Western blotting methods are described.