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    • 玉米低聚肽改善地塞米松诱导的肌肉萎缩效果研究

    WANG Yunfeng,YAN Jiuming,ZHOU Zhengming,GUO Jiankui,WEI Yaoyao,JING Xiaofan,LIAO Xinyi,HU Wen

    West China Medical Journal（2023）

    四川大学华西公共卫生学院；四川大学华西第四医院 | 四川大学华西医院 | 四川大学

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    Abstract
    目的 研究玉米低聚肽(corn oligopeptide,COP)对地塞米松诱导的肌肉萎缩的改善作用.方法 49只8周龄Sprague-Dawley雄性大鼠分为空白组(10只)和模型组(39只),模型组腹腔注射地塞米松(1.0mg/kg),空白组注射生理盐水.19 d后,空白组取1只、模型组取3只观察模型是否构建成功.造模成功后,将模型组随机分为模型对照组、COP低剂量组(COP-L组,0.5 g/kg)、COP中剂量组(COP-M组,1.0 g/kg)和COP高剂量组(COP-H组,2.0g/kg),9只/组,干预33d后测大鼠抓力,随后麻醉处死,分离腓肠肌、比目鱼肌、胫骨前肌和跖肌称重,并测肌纤维直径、Atrogin-1和MuRF-1 mRNA相对表达量及腓肠肌非靶向代谢组学.结果 与空白组相比,模型组大鼠体重显著降低(P＜0.05),肌原纤维破裂,提示造模成功.与模型对照组相比,COP-L组、COP-M组抓力提高(P＜0.05);COP-L组、COP-H组腓肠肌、比目鱼肌肌肉系数增大(P＜0.05),COP-L组、COP-M组跖肌肌肉系数增大(P＜0.05);COP 3个剂量组大鼠胫骨前肌肌纤维直径增加(P＜0.05),COP-M组、COP-H组跖肌肌纤维直径增加(P＜0.05);COP 3个剂量组比目鱼肌Atrogin-1 mRNA相对表达量均下降(P＜0.05),COP-L组、COP-H组MuRF-1 mRNA相对表达量下降(P＜0.05).腓肠肌中氨基酸合成通路、糖酵解通路、酸代谢通路激活.结论 给予COP可显著改善地塞米松诱导的肌肉萎缩,其机制可能与COP减小泛素-蛋白酶体途径中关键基因Atrogin-1、MuRF-1表达和增加肌肉中重要氨基酸的生物合成有关.
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    【要点】：研究玉米低聚肽对地塞米松诱导的肌肉萎缩的改善作用及其机制。
    

    【方法】：采用腹腔注射地塞米松构建肌肉萎缩模型，给予不同剂量的玉米低聚肽进行干预，通过测量大鼠抓力、肌肉系数、肌纤维直径及mRNA表达量等指标评估改善效果。
    

    【实验】：实验使用49只Sprague-Dawley雄性大鼠，分为空白组和模型组。模型组通过腹腔注射地塞米松构建肌肉萎缩模型，随后分为模型对照组及不同剂量的COP处理组，干预33天后进行各项指标的测量，使用的数据集名称未在文中提及。结果显示，COP能够显著提高抓力、肌肉系数及肌纤维直径，并降低肌肉萎缩相关基因表达，提示COP具有改善地塞米松诱导的肌肉萎缩的作用。