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    • Synthesis of Single-Walled Carbon Nanotubes Functionalized with Platinum Nanoparticles to Sense Breast Cancer Cells in 4T1 Model to X-ray Radiation.

    Afsoon Aghaei,Maryam Shaterian,Hossein Danafar,Blaž Likozar,Andraž Šuligoj,Sašo Gyergyek

    Microchimica Acta（2023）

    Faculty of Science | Zanjan University of Medical Sciences | National Institute of Chemistry | Jozef Stefan Institute

    Cited 3|Views12
    Abstract
    In recent years, various types of radiosensitizers have been developed to address the challenges of cancer radiotherapy. Here, platinum-functionalized oxygenated single-walled carbon nanotubes (O-SWCNTs-Pt) coated with folic acid (FA) and bovine serum albumin (BSA) (O-SWCNTs-Pt-BSA-FA) were synthesized, characterized, and used as radiosensitizers to improve the therapeutic efficacy of X-rays in a mouse model of breast cancer (4T1) in vitro. The nanosensitizer was characterized by different techniques, such as transmission electron microscopy (TEM), selected area electron diffraction (SAED), dynamic light scattering (DLS), zeta potential, X-ray diffraction (XRD), ultraviolet–visible (UV-visible), and Fourier transform infrared (FTIR) spectrometry. The evaluation of cell viability with nanocarriers O-SWCNTs-BSA, O-SWCNTs-Pt-BSA, Pt-BSA-FA, and O-SWCNTs-Pt-BSA-FA is reported at the concentrations of 10, 30, and 90 μg/mL by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in the presence and absence of X-rays at 4 and 8 Gy. The results showed that administration of O-SWCNTs-BSA, O-SWCNTs-Pt-BSA, Pt-BSA-FA, and O-SWCNTs-Pt-BSA-FA + 8 Gy at a concentration of 90 μg/mL reduced survival by 75.31, 65.32, 67.35, and 60.35
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    Key words
    Radiosensitizers,Single-walled carbon nanotubes,Platinum,Bovine serum albumin,Folic acid,MTT assay
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    【要点】：该论文合成了铂功能化的氧化单壁碳纳米管（O-SWCNTs-Pt）并将其与叶酸（FA）和牛血清白蛋白（BSA）结合，用于识别并增强4T1乳腺癌模型对X射线辐射的放射增敏效果。
    

    【方法】：通过透射电子显微镜（TEM）、选区电子衍射（SAED）、动态光散射（DLS）、zeta电位、X射线衍射（XRD）、紫外-可见（UV-visible）和傅里叶变换红外（FTIR）光谱等技术对纳米增敏剂进行了表征。
    

    【实验】：使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴化物（MTT）法评估了在4T1乳腺癌细胞系中，不同浓度的O-SWCNTs-BSA、O-SWCNTs-Pt-BSA、Pt-BSA-FA和O-SWCNTs-Pt-BSA-FA对细胞活力的影响，并在有和无X射线（4和8戈瑞）的条件下进行了实验。结果显示，在90μg/mL浓度下，这些纳米载体与8戈瑞X射线联合使用时，生存率分别减少了75.31%、65.32%、67.35%和60.35%。O-SWCNTs-Pt-BSA-FA具有88.57纳米的 hydrodynamic size 和 -29 mV 的表面电荷，表明其具有特殊的稳定性。与O-SWCNTs-BSA、O-SWCNTs-Pt-BSA和Pt-BSA-FA相比，在4T1细胞系中它具有非常强的细胞杀伤活性。值得注意的是，SWCNTs可以作为PtNPs的控释和递送系统，因为它们具有独特的性质并且容易穿透生物膜。因此，这种新的纳米增敏剂可能会与放射治疗技术一起在癌症治疗中发挥作用。