Chrome Extension
WeChat Mini Program
Use on ChatGLM
VIPCreated with Sketch.

    • Synthesis of Single-Walled Carbon Nanotubes Functionalized with Platinum Nanoparticles to Sense Breast Cancer Cells in 4T1 Model to X-ray Radiation.

    Afsoon Aghaei,Maryam Shaterian,Hossein Danafar,Blaž Likozar,Andraž Šuligoj,Sašo Gyergyek

    Mikrochimica Acta（2023）

    Faculty of Science | Zanjan University of Medical Sciences | National Institute of Chemistry | Jozef Stefan Institute

    Cited 3|Views12
    Abstract
    In recent years, various types of radiosensitizers have been developed to address the challenges of cancer radiotherapy. Here, platinum-functionalized oxygenated single-walled carbon nanotubes (O-SWCNTs-Pt) coated with folic acid (FA) and bovine serum albumin (BSA) (O-SWCNTs-Pt-BSA-FA) were synthesized, characterized, and used as radiosensitizers to improve the therapeutic efficacy of X-rays in a mouse model of breast cancer (4T1) in vitro. The nanosensitizer was characterized by different techniques, such as transmission electron microscopy (TEM), selected area electron diffraction (SAED), dynamic light scattering (DLS), zeta potential, X-ray diffraction (XRD), ultraviolet–visible (UV-visible), and Fourier transform infrared (FTIR) spectrometry. The evaluation of cell viability with nanocarriers O-SWCNTs-BSA, O-SWCNTs-Pt-BSA, Pt-BSA-FA, and O-SWCNTs-Pt-BSA-FA is reported at the concentrations of 10, 30, and 90 μg/mL by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in the presence and absence of X-rays at 4 and 8 Gy. The results showed that administration of O-SWCNTs-BSA, O-SWCNTs-Pt-BSA, Pt-BSA-FA, and O-SWCNTs-Pt-BSA-FA + 8 Gy at a concentration of 90 μg/mL reduced survival by 75.31, 65.32, 67.35, and 60.35
    More
    Translated text
    Key words
    Radiosensitizers,Single-walled carbon nanotubes,Platinum,Bovine serum albumin,Folic acid,MTT assay
    求助PDF
    上传PDF
    View via Publisher
    Bibtex
    AI Read Science
    AI Summary
    AI Summary is the key point extracted automatically understanding the full text of the paper, including the background, methods, results, conclusions, icons and other key content, so that you can get the outline of the paper at a glance.
    Example
    Background
    Key content
    Introduction
    Methods
    Results
    Related work
    Fund
    Key content
    • Pretraining has recently greatly promoted the development of natural language processing (NLP)
    • We show that M6 outperforms the baselines in multimodal downstream tasks, and the large M6 with 10 parameters can reach a better performance
    • We propose a method called M6 that is able to process information of multiple modalities and perform both single-modal and cross-modal understanding and generation
    • The model is scaled to large model with 10 billion parameters with sophisticated deployment, and the 10 -parameter M6-large is the largest pretrained model in Chinese
    • Experimental results show that our proposed M6 outperforms the baseline in a number of downstream tasks concerning both single modality and multiple modalities We will continue the pretraining of extremely large models by increasing data to explore the limit of its performance
    Upload PDF to Generate Summary
    Must-Reading Tree
    Example
    Generate MRT to find the research sequence of this paper
    Data Disclaimer
    The page data are from open Internet sources, cooperative publishers and automatic analysis results through AI technology. We do not make any commitments and guarantees for the validity, accuracy, correctness, reliability, completeness and timeliness of the page data. If you have any questions, please contact us by email: report@aminer.cn
    Chat Paper
    【要点】：该论文合成了铂功能化的氧化单壁碳纳米管（O-SWCNTs-Pt）并将其与叶酸（FA）和牛血清白蛋白（BSA）结合，用于识别并增强4T1乳腺癌模型对X射线辐射的放射增敏效果。
    

    【方法】：通过透射电子显微镜（TEM）、选区电子衍射（SAED）、动态光散射（DLS）、zeta电位、X射线衍射（XRD）、紫外-可见（UV-visible）和傅里叶变换红外（FTIR）光谱等技术对纳米增敏剂进行了表征。
    

    【实验】：使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴化物（MTT）法评估了在4T1乳腺癌细胞系中，不同浓度的O-SWCNTs-BSA、O-SWCNTs-Pt-BSA、Pt-BSA-FA和O-SWCNTs-Pt-BSA-FA对细胞活力的影响，并在有和无X射线（4和8戈瑞）的条件下进行了实验。结果显示，在90μg/mL浓度下，这些纳米载体与8戈瑞X射线联合使用时，生存率分别减少了75.31%、65.32%、67.35%和60.35%。O-SWCNTs-Pt-BSA-FA具有88.57纳米的 hydrodynamic size 和 -29 mV 的表面电荷，表明其具有特殊的稳定性。与O-SWCNTs-BSA、O-SWCNTs-Pt-BSA和Pt-BSA-FA相比，在4T1细胞系中它具有非常强的细胞杀伤活性。值得注意的是，SWCNTs可以作为PtNPs的控释和递送系统，因为它们具有独特的性质并且容易穿透生物膜。因此，这种新的纳米增敏剂可能会与放射治疗技术一起在癌症治疗中发挥作用。