Cofunctioning of bacterial exometabolites drives root microbiota establishment
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA(2023)
摘要
Soil-dwelling microbes are the principal inoculum for the root microbiota, but our under-standing of microbe-microbe interactions in microbiota establishment remains fragmen-tary. We tested 39,204 binary interbacterial interactions for inhibitory activities in vitro, allowing us to identify taxonomic signatures in bacterial inhibition profiles. Using genetic and metabolomic approaches, we identified the antimicrobial 2,4-diacetylphloroglucino l (DAPG) and the iron chelator pyoverdine as exometabolites whose combined func-tions explain most of the inhibitory activity of the strongly antagonistic Pseudomonas brassicacearum R401. Microbiota reconstitution with a core of Arabidopsis thaliana root commensals in the presence of wild -type or mutant strains revealed a root niche-specific cofunction of these exometabolites as root competence determinants and drivers of pre-dictable changes in the root-associated community. In natural environments, both the corresponding biosynthetic operons are enriched in roots, a pattern likely linked to their role as iron sinks, indicating that these cofunctioning exometabolites are adaptive traits contributing to pseudomonad pervasiveness throughout the root microbiota.
更多查看译文
关键词
root microbiome, microbe-microbe interactions, synthetic ecology, competition, secondary metabolites
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要