Molecular Detection of SARS-CoV-2 in Argentina: Evaluation of Alternative Diagnostic Tools for the Decentralization of the Diagnosis

The rocketing number of COVID-19 cases highlighted the critical role that diag-nostic tests play in medical and public health decision-making to contain and mitigate the SARS-CoV-2 pandemic. This study reports the evaluation and implementation of different tests for the molecular detection of SARS-CoV-2 in the central region of Argentina. We evaluated 3 real time RT-PCR kits (GeneFinder COVID-19 Plus RealAmp Kit, DisCoVery SARS-CoV-2 RT-PCR Detection Kit and WGene SARS-CoV-2 RT Detection), 2 nucleic acid extraction methods [MagaBio plus Virus DNA/RNA Purification Kit II (BioFlux), 35-min vs. 9-min], a pre-analytical reagent (FlashPrep (R)) and 2 isothermal amplification tests (Neokit Plus and ELA CHEMSTRIP (R)). The order according to the best performance of the 3 real-time RT-PCR kits evaluated was: Dis-CoVery > GeneFinderTM > WGene. The 2 RNA extraction methods showed similar good results: MagaBio plus Virus RNA Purification Kit II (BioFlux) 9-min was selected due to its faster per-formance. FlashPrep (R) reagent showed excellent results to perform direct RNA detection. Isothermal amplification assays showed acceptable sensitivity and specificity values (>80%), except in samples with Ct > 30. Our data show optimal real time RT-PCR kits and alternative molecular methods for SARS-CoV-2 diagnostic. These alternative assays proved to be acceptable for their use in adverse contexts, decentralization, and different epidemiological scenarios, for rapid and accurate SARS-CoV-2 detection. (c) 2023 Asociacion Argentina de Microbiologi ' a. Published by Elsevier Espana, S.L.U.