Supplementary Table 1, Figures 1 - 8 from Cisplatin Resistance Associated with PARP Hyperactivation

PDF file - 490K, Supplementary Table S1. Systematic comparison of the effects of CDDP and PARP inhibitors on cancer cell lines used in this study. Supplementary Figure S1. Generation and characterization of CDDP-resistant human NSCLC cell clones. Supplementary Figure S2. Effects of CDDP and PARP inhibitors on the intracellular levels of poly(ADPribosyl) ated proteins in WT and CDDP-resistant NSCLC cells. Supplementary Figure S3. DNA damage as induced by PARP inhibitors is not a consequence of apoptotic caspase activation. Supplementary Figure S4. Markers of homologous recombination (HR) in CDDP-sensitive and CDDP-resistant A549 cells responding to PARP inhibitors. Supplementary Figure S5. DNA repair activity of A549 cell extracts towards dA�T- and THF�T-containing substrates. Supplementary Figure S6. DNA glycosylase activity of A549 cell extracts towards Hx�T- and U�G-containing substrates. Supplementary Figure S7. DNA repair activity of A549 cell extracts towards 5-OHC�T-and 8oxoG�C-containing substrates. Supplementary Figure S8. Expression levels and functional activity of XRCC1 and POL in CDDP-sensitive and CDDP-resistant cancer cells that overexpress PARP1 and contain high levels of poly(ADP-ribosyl)ated proteins.