Supplementary Figure 2 from Immune Response Is an Important Aspect of the Antitumor Effect Produced by a CD40L-Encoding Oncolytic Adenovirus

PDF file - 2.2MB, (A) Cell monolayers were infected with 100 viral paticles /cell (VP/cell) and 24 hours later cells were analyzed by flow-cytometry measuring Annexin-V expression. Results are presented as percentage of positive cells relative to Mock uninfected cells. **, P<0.01. (B) CD40 expression is shown in brown in A549 and EJ tumors. Pictures were taken with an Axioplan Microscope equipped with a camera Axiocam at a magnification of 10x. (C) Tumors from nude mice bearing either A549 tumors (left panel) or EJ tumors (right panel) injected with viruses were analyzed for viral load. Quantitative PCR was performed for E4 sequence and values were normalized to human beta-actin gene.. The real-time PCR conditions were as described in (31). All samples were tested in duplicate. Results are presented as mean from all mice +SEM. **, P<0.01.