Why proanthocyanidins elute at increasing order of molecular masses when analysed by normal phase high performance liquid chromatography? Considerations of use

Esperanza Guerrero-Hurtado,Alba Gutiérrez-Docio, Rebeca Fiedorowicz, Esperanza Mollá,Guillermo Reglero,Marin Prodanov

Journal of Chromatography A（2023）

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摘要

•Proanthocyanidin assessment by precipitation/redissolution HPLC.•NP-HPLC separates proanthocyanidins by a precipitation/redissolution mechanism.•NP-HPLC separates grape seed monomeric phenols by adsorption/partition mechanism.•Guidelines for reproducible use of NP-HPLC in proanthocyanidin analysis.

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关键词

Proanthocyanidins,Grape seed extract,NP-HPLC,Solvent/non-solvent system,Precipitation/redissolution HPLC

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