Construction and application of the genome-scale metabolic model of Streptomyces radiopugnans .

Geosmin is one of the most common earthy-musty odor compounds, which is mainly produced by . was screened in radiation-polluted soil, which has the potential to overproduce geosmin. However, due to the complex cellular metabolism and regulation mechanism, the phenotypes of were hard to investigate. A genome-scale metabolic model of named ZDZ767 was constructed. Model ZDZ767 involved 1,411 reactions, 1,399 metabolites, and 767 genes; its gene coverage was 14.1%. Model ZDZ767 could grow on 23 carbon sources and five nitrogen sources, which achieved 82.1% and 83.3% prediction accuracy, respectively. For the essential gene prediction, the accuracy was 97.6%. According to the simulation of model ZDZ767, D-glucose and urea were the best for geosmin fermentation. The culture condition optimization experiments proved that with D-glucose as the carbon source and urea as the nitrogen source (4 g/L), geosmin production could reach 581.6 ng/L. Using the OptForce algorithm, 29 genes were identified as the targets of metabolic engineering modification. With the help of model ZDZ767, the phenotypes of could be well resolved. The key targets for geosmin overproduction could also be identified efficiently.