Current and Emerging Diagnostic Approaches to Bacterial Diseases of Ruminants.
The Veterinary clinics of North America Food animal practice(2023)
Abstract
The diagnostic approaches and methods to detect bacterial pathogens in ruminants are discussed, with a focus on cattle. Conventional diagnostic methods using culture, isolation, and characterization are being replaced or supplemented with new methods. These include molecular diagnostics such as real-time polymerase chain reaction and whole-genome sequencing. In addition, methods such as matrix-assisted laser desorption ionization-time-of-flight mass spectrometry enable rapid identification and enhanced pathogen characterization. These emerging diagnostic tools can greatly enhance the ability to detect and characterize pathogens, but performance and interpretation vary greatly across sample and pathogen types, disease syndromes, assay performance, and other factors.
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Key words
Bovine,Diagnostics,Bacterial pathogens,MALDI-TOF,Real-time PCR,Bovine respiratory disease,Mastitis,Abortion,Bovine keratoconjunctivitis
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