P14 New transcriptional alterations in the pathogenesis of type 2 diabetes, its potential regulation by micro RNAs

Introduction: β cell dysfunction is conditioned by gene expression alterations and their regulatory mechanisms. Micro RNAs (miRNAs) are small noncoding RNAs that regulate gene expression by interacting and targeting messenger RNA (mRNAs). An adequate integration of mRNA and miRNA expression profiles facilitates elucidation of significant biological alterations. Aim: to identify new transcriptomic alterations and miRNAs involved in type 2 diabetes (T2D) pathogenesis through the integration of data available in public databases. Materials and methods: 1. 7 microarray studies performed from islets of people without diabetes (ND; n = 245) and with T2D (n = 96) were analyzed. For further meta-analysis, we identified Differentially Expressed Genes (DEGs) (ND vs. DT2) and selected those that varied consistently in the different studies. 2. mRNA and miRNA expression profiles were integrated, and computationally predicted miRNA-mRNA interactions to find dysregulated miRNAs relevant to T2D pathogenesis. This analysis was performed using a study from a T2D murine model in which both types of RNAs were analyzed in the same pancreatic tissue, using Next Generation Sequencing techniques.