Isoorientin Ameliorates Osteoporosis and Oxidative Stress in Postmenopausal Rats

Context Isoorientin has many biological activities, including antioxidant, anti-inflammatory, antitumor. However, the effect of isoorientin on postmenopausal osteoporosis remains unclear. Objective To evaluate the effect of isoorientin on postmenopausal osteoporosis. Materials and methods Sprague-Dawley rats were divided into five groups (n = 5): sham, model, 17-beta-oestradiol (E2, 10 mu g/kg/day), low-dose isoorientin (L-Iso, 50 mg/kg), and high-dose isoorientin (H-Iso, 100 mg/kg). The rats were ovariectomized, treated by gavage daily for 12 weeks, and serum and femur samples were collected. Bone mineral density, bone metabolism, and oxidative stress were assessed. H&E staining, immunohistochemistry, and western blotting were employed. Results Isoorientin improved the bone mineral density of the lumbar vertebrae (2.01 +/- 0.05 g/cm(3) in H-Iso group vs. 1.74 +/- 0.07 g/cm(3) in model group) and femur (1.46 +/- 0.06 g/cm(3) vs. 1.19 +/- 0.03 g/cm(3)), increased the trabecular bone number (1.97 +/- 0.03 vs. 1.18 +/- 0.13) and thickness (0.27 +/- 0.02 vs. 0.16 +/- 0.03 mm). Isoorientin decreased the separation degree of trabecular bone, ameliorated bone histomorphology changes, and significantly improved the mechanical properties. Isoorientin diminished MDA (by 60%) and increased SOD (by 49.2%), and GSH-Px (by 159%) activity. Furthermore, osteoprotegerin (OPG), nuclear factor erythroid 2-like 2 (Nrf2), haem oxygenase (HO-1), NAD(P)H quinone dehydrogenase 1(NQO1), and oestrogen receptor 1(ESR1) protein expression increased, while receptor activator of nuclear factor-kappa B ligand (RANKL) protein expression decreased after treatment. Conclusions Isoorientin ameliorates osteoporosis via upregulating OPG and Nrf2/ARE signalling, suggesting isoorientin maybe a potential therapeutic drug for PMOP.