MDR Isolates Virulence of DNase activity Identified Staphylococcus and Pseudomonas Species from Diabetic Foot Ulcer

Diabetes mellitus is the leading cause of death and morbidity from long-term consequences. Antibiotic resistance is the most common treatment for diabetic foot ulcers. Chronic wounds are caused by antibiotic misuse, mistreatment of medications, and a reduction in the effectiveness of effective antibiotics in the wound or infection environment. Chemical suppression of bacterial intercellular communication, particularly quorum sensing, is of particular relevance in this regard because it regulates pathogenicity in many therapeutically relevant infections where resistance to antibiotics is a challenge. Resistance genes are also acquired through the interchange of bacterial plasmids and chromosomal. Our aim and objective is to microbiological culture study for the clinical pus samples of foot diabetic patients. Our study comprised 131 samples from diabetic foot ulcer patients, with male patients providing for 59% and female patients accounting for 41%. In 131 sample progressing reports, 81 isolates were discovered, ranging in age from 40 to 80 years. The most commonly infected age groups are 51 to 60, with the majority of infection cases categorized as grade III. A sample was taken. The sample grew 97 percent of the time and grew just 3% of the time, with mono microbial growth 48 percent of the time and poly microbial growth 49 percent of the time. MDR Gram positive staphylococci 14 isolates and gram negative pseudomonas 11 isolates showed resistance to 25 culture DNase enzyme activity. In staphylococci 9 isolates and pseudomonas 6 isolates, the DNase enzyme activity in DNase agar media light zone formed. In conclusion diabetic foot ulcers are one of the most prevalent, expensive, and serious consequences of diabetes, and they are considered a major public health issue. Early diagnosis and successful therapy require accurate identification of the causative microorganisms.