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A New Strategy for Increasing Knock-in Efficiency: Multiple Elongase and Desaturase Transgenes Knock-in by Targeting Long Repeated Sequences.

De Xing,Shangjia Li,Mei Shang,Wenwen Wang, Qin Zhang,Jinhai Wang,Tasnuba Hasin,Darshika Hettiarachchi,Veronica Alston,Logan Bern,Abel Paladines Parrales,Cuiyu Lu,Michael Coogan,Andrew Johnson,Zhenkui Qin,Baofeng Su,Rex Dunham

ACS Synthetic Biology（2022）

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关键词
CRISPR,Cas9,knock-in efficiency,long repeated sequence,fatty acids
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