Molecular Testing Opportunities on Cytology Effusion Specimens: the Pre-Analytic Effects of Various Body Fluid Cytology Preparation Methods on RNA Extraction Quality and Targeted Sequencing.

•Routine cytologic slide preparations can produce RNA sequencing results that are superior to formalin-fixed paraffin-embedded cell block preparations.•Cytology effusion specimens can be a source of high quantity and quality material for RNA sequencing analysis.•This information is critically important as cancer treatments are trending towards personalized, targeted therapies.