Assays for the Enzymes Catalyzing the O-Acetylation of Bacterial Cell Wall Polysaccharides
Methods in Molecular Biology(2019)
摘要
The polysaccharides that comprise bacterial cell walls are commonly O-acetylated. This modification confers resistance to hydrolases of innate immune systems and/or controls endogenous autolytic activity. Herein, we present protocols for the compositional analysis of bacterial cell wall O-acetylation, and assays for monitoring O-acetyltransferases and O-acetylesterases. The assays are amenable for the development of high-throughput screens in search of inhibitors of the respective enzymes.
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关键词
O-Acetylation,O-acetyltransferase,O-acetylesterase,Peptidoglycan,Chitooligosaccharides
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