Macrocycle molecule-based cryoprotectants for ice recrystallization inhibition and cell cryopreservation

Cyclodextrin-based cryoprotectants were developed. alpha-TMCD, which can be easily put into large-scale production, showed enhanced cell viabilities of 19.97 +/- 0.78%, 13.93 +/- 4.46% and 19.10 +/- 0.95% against GES-1, hucMSCs and A549 cells. Moreover, the viable cells observed by light microscope imaging showed that the enhanced hucMSC cell number percentage of alpha-TMCD was 103.2%. An alpha-TMCD-DMSO-based CPA exhibited an enhanced cryoprotective effect by a mechanism of DMSO-enhanced cell penetrating effect and alpha-TMCD-DMSO synergistically enhanced IMA ability. alpha-TMCD exhibited potential for the discovery of macrocycle-molecule-based cryoprotectants.