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Monitoring the Progression of Cell-Free Expression of Microbial Rhodopsins by Surface Enhanced IR Spectroscopy: Resolving a Branch Point for Successful/Unsuccessful Folding

FRONTIERS IN MOLECULAR BIOSCIENCES(2022)

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Key words
FTIR-spectroscopy,membrane protein folding,cell-free expression,SEIRA (surface enhanced infrared absorption),sensory rhodopsin,channelrhodopsin,co-translational folding
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