Stress responses in camels subjected to different rest periods (0 and 12 h) at slaughterhouse

This study investigated the effect of two different rest periods (R0: no rest time, and R12: 12 h of rest) on several stress indicators, including plasma and serum stress parameters, in dromedary camels (Camelus dromedarius). This study involved 60 healthy male dromedary camels aged 5 years, from which blood samples were collected. Cortisol, glucose, urea, creatinine, the enzymatic activity of creatine kinase, lactic dehydrogenase, aspartate aminotransferase, alanine aminotransferase, and alkalinephosphatase were analyzed. Data obtained indicated that rest time had a significant effect on cortisol, creatinine, creatine kinase, lactic dehydrogenase and aspartate aminotransferase (p < 0.05). However, no statistically significant effect was found for glucose, urea, alanine aminotransferase, and alkalinephosphatase. The values of cortisol and creatinine showed a significant decrease at R12. In contrast, the enzymatic activity of creatine kinase, lactic dehydrogenase, and aspartate aminotransferase, significantly increased at R12. Additionally, several correlations between different studied parameters were documented, including for lactic dehydrogenase. The results obtained in this study suggest that rest time may influence welfare indicating parameters. In summary, a rest time of more than 12 h is recommended in order to allow camels to recover from the effects of pre-slaughter stress. Additionally, lactate dehydrogenase might be suitable to use as an indicator of stress in camels during the slaughter process.