Protective Effects of Feruloyl Oligosaccharides from Fermented Wheat Bran against Oxidative Stress in IPEC-J2 Cells In Vitro and in a Zebrafish Model In Vivo

This study aims to evaluate the protective effects of feruloyl oligosaccharides from fermented wheat bran (FOs-FWB) against oxidative stress in IPEC-J2 cells in vitro and in a zebrafish model in vivo. Results showed that FOs-FWB effectively reduces reactive oxygen species (ROS) and malondialdehyde (MDA) content in lipopolysaccharide (LPS)-stimulated IPEC-J2 cells while elevating glutathione (GSH) content and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities. The real-time PCR data illustrated that FOs-FWB upregulated the mRNA expression levels of glutamate-cysteine ligase catalytic subunit (GCLC), glutamate-cysteine ligase modifier subunit (GCLM), NAD (P) H: quinone oxidoreductase-1 (NQO-1), and heme oxygenase-1 (HO-1). In the model of AAPH-stimulated zebrafish embryos, we observed that FOs-FWB suppressed cell death, ROS generation, and lipid peroxidation, along with improvements in SOD, CAT, and GSH-Px activities. Therefore, FOs-FWB exerted protective effects against oxidative stress in IPEC-J2 cells and zebrafish.