Validation of a GC- and LC-MS/MS based method for the quantification of 22 estrogens and its application to human plasma

Carolin Kleider, Jeniffer Calderón Giraldo,Daniela Pemp,Harald L Esch,Leane Lehmann

Steroids（2022）

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摘要

•Stable-isotope dilution method for the analysis of 22 estrogens in human plasma.•Application to plasmas (1 ml) of healthy pre- and postmenopausal women.•Estradiol and estrone and their conjugates prevailed in human plasma.•Among oxidative metabolites, only 2-methoxyestrone and its glucuronide were found.

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关键词

E1,E2,G,GC,(U)HPLC,HO,LC,LOD,LOQ,MeO,MRM,MS/MS,m/z,SPE,S

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