HPLC ANALYSIS OF AMINO ACIDS CONTENT IN CRAMBE CORDIFOLIA AND CRAMBE KOKTEBELICA LEAVES

Objective: The aim of our study was to establish the content of some primary metabolites, such as amino acids in Crambe cordifolia and Crambe koktebelica. The lack of experimental data induced us to determine these compounds. Methods: Crambe cordifolia and Crambe koktebelica leaves were selected as the objects of the study. The amino acids in the raw materials were determined by the HPLC method. Results: The results of the research revealed that the leaves of Crambe cordifolia and Crambe koktebelica contain fifteen and sixteen free amino acids respectively. Among the free amino acids L-histidine was presented in Crambe cordifolia leaves in the greatest amount, its content was 12.19 µg/mg. The content of free L-arginine, L-valine, L-phenylalanine, L-isoleucine was the greatest in Crambe koktebelica leaves, it was 2.23 µg/mg, 2.04 µg/mg, 1.74 µg/mg, 1.50 µg/mg respectively. The content of bound L-glutamic acid, Glycine, L-arginine, L-leucine was the highest in Crambe cordifolia and Crambe koktebelica leaves. Conclusion: The results of the study showed that Crambe cordifolia and Crambe koktebelica can be considered as a source of highly digestible amino acids that can be used to treat some diseases.