Isolation of Fusarium Asiaticum from Creeping Bentgrass with Blight Symptom and Its Trichothecene Chemotype

We identified the causal agent of the blight disease of creeping bentgrass. When a representative isolate, #B-s1, reinfected the plant, the same fungus was reisolated from the diseased plant tissue. The pathogen was identified as Fusarium asiaticum by colony characteristics and sequence analysis. The isolate produced nivalenol on solid rice medium, which was detected by high performance liquid chromatography. In addition, all other isolates were determined as nivalenol chemotype of F. asiaticum by diagnostic PCR.