茄子根际促生菌筛选鉴定及其促生效应

ZHANG Zhi,ZHANG Yao,WANG Zhigang,XU Weihui

Journal of Science of Teachers' College and University（2022）

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Abstract

以齐齐哈尔龙江县茄子根际土壤为实验材料,采用稀释平板法筛选根际促生菌(plant growth-promoting rhizobacteria,PGPR),通过16S rRNA核苷酸序列分析鉴定菌种,探究菌株的生理活性,并进行安全性评价;通过浸种和盆栽实验研究菌株对茄子种子萌发和幼苗生长的影响.结果表明,在茄子根际筛选到7株PGPR,其分别属于Bacillussp.QJ1,Chryseobacterium aquifrigidense QJ2,Bacillus megaterium QJ4,Enterobacter aerogenes NQ1△,Bacterium NQ1,Bacterium NQ3,Enterobactersp.NQ5;不同的菌株溶磷、分泌赤霉素和吲哚乙酸(IAA)能力不同;菌株产生铁载体,仅有NQ3菌株具有溶血性.浸种和盆栽实验表明,NQ1△菌悬液显著促进茄子种子的萌发,QJ2菌悬液对茄子幼苗生长有显著的促进作用.综上所述,菌株NQ1△,QJ2可分别作为茄子种子包衣及专用促生肥料的候选菌剂.

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