Quantitative evaluation of intercellular local deformation of human oocytes during Piezo-assisted intracytoplasmic sperm injection using video-based motion analysis

The Piezo-ICSI process induced deformation with various mechanical behaviors in different regions within a single oocyte. Furthermore, the exposure of the ooplasm adjacent to the first polar body to excessive deformation was found to be a possible trigger for adverse fertilization and embryo development after Piezo-ICSI.