Antibody Assays in seropositive subjects

3 Kahina Saker, Bruno Pozzetto, Vanessa Escuret, Virginie Pitiot, Amélie Massardier4 Pilonchéry, Bouchra Mokdad, Carole Langlois-Jacques, Muriel Rabilloud, Dulce 5 Alfaiate, Nicolas Guibert, Jean-Baptiste Fassier, Antonin Bal, Sophie Trouillet-Assant, 6 Mary-Anne Trabaud. 7 8 Laboratoire de Virologie, Institut des Agents Infectieux, Laboratoire associé au Centre 9 National de Référence des virus des infections respiratoires, Hospices Civils de Lyon, IAI, 10 Centre de Biologie Nord, Groupement Hospitalier Nord, F-69317, Lyon Cedex 04, France 11 CIRIInternational Center of Research in Infectiology, INSERM U1111, CNRS UMR5308, 12 ENS Lyon, Claude Bernard Lyon 1 University, F-69008, Lyon, France. 13 Occupational Health and Medicine Department, Hospices Civils de Lyon, Lyon, France 14 CNRS, UMR 5558, University of Lyon, Laboratoire de Biométrie et Biologie Evolutive, 15 Equipe Biostatistique-Santé, F-69100, Villeurbanne, France 16 17 18 ABSTRACT 19 The virus neutralization test (VNT) is the reference for the assessment of the functional ability 20 of neutralizing antibodies (NAb) to block SARS-CoV-2 entry into cells. New competitive 21 immunoassays measuring antibodies preventing interaction between the spike protein and its 22 cellular receptor are proposed as surrogate VNT (sVNT). We tested three commercial sVNT 23 (a qualitative immunochromatographic test and two quantitative immunoassays named YHLO 24 . CC-BY-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. (which was not certified by peer review) The copyright holder for this preprint this version posted April 1, 2022. ; https://doi.org/10.1101/2022.01.04.22268652 doi: medRxiv preprint