Production and Partial Characterization of Alkaline Polygalacturonase Secreted by Thermophilic Bacillus Sp Smia-2 under Submerged Culture Using Pectin and Corn Steep Liquor

Polygalacturonase production by the thermophilic Bacillus sp. SMIA-2 cultivated in liquid cultures containing 0.5% (w/v) apple pectin and supplemented with 0.3% (w/v) corn steep liquor, reached its maximum after 36 hours with levels of 39 U.mL(-1). The increase in apple pectin and corn steep liquor concentrations in the medium from 0.5 and 0.3%, respectively, to 0.65%, markedly affected the production of polygalacturonase, whose activity increased four times, reaching a maximum of 150.3 U.mL(-1). Studies on polygalacturonase characterization revealed that the optimum temperature of this enzyme was between 60-70 degrees C. Thermostability profile indicated that the enzyme retained about 82 and 63% of its activity at 60 and 70 degrees C, respectively, after 2 hours of incubation. The optimum pH of the enzyme was found to be 10.0. After incubation of crude enzyme solution at room temperature for 2 hours at pH 8.0, a decrease of about 29% on its original activity was observed. At pH 10.0, the decrease was 25%.